Compound Overview

B12 (Cyanocobalamin) is a cobalamin derivative and cobalt-containing corrinoid with the molecular formula C₆₃H₈₈CoN₁₄O₁₄P. It is the cyano form of cobalamin and is distinguishable from other cobalamin forms in compound-specific analytical workflows.

This material is supplied as a laboratory research reagent intended for controlled experimental environments, including analytical method development, reference standard work, and structural or binding studies involving cobalamin transport proteins.

Molecular Information

Compound Type: Cobalamin derivative
Molecular Formula: C₆₃H₈₈CoN₁₄O₁₄P
Molecular Weight: 1355.37 g/mol
CAS Number: 68-19-9
Purity: 99.80%

Research Applications

Published research uses cyanocobalamin in quantitative analytical workflows and as a defined ligand in protein-binding and transporter structure studies. Compound-specific reports describe its qualification as a reference standard, chromatographic and spectrometric determination in reference materials and complex matrices, and structural characterization in complexes with bacterial cobalamin transport proteins.

Preparation and qualification of cyanocobalamin reference standards by infrared spectroscopy, thin-layer chromatography, HPLC, and assay testing
HPLC-based quantitation of cyanocobalamin in standard reference materials and multicomponent tablet matrices
LC/ICP-MS determination of cyanocobalamin through chromatographic separation and cobalt-specific detection
Stable isotope dilution LC-MS/MS methods that separately quantify cyanocobalamin alongside other cobalamin forms
UV-Vis spectrophotometric method development and validation using cyanocobalamin absorbance measurements
Ligand-bound structural studies of bacterial cobalamin transport proteins such as BtuB and BtuM