Compound Overview

Glutathione is a tripeptide thiol composed of glutamate, cysteine, and glycine. Its structure is defined by a _-glutamyl linkage between the glutamate residue and cysteinylglycine.

This material is supplied as a laboratory research reagent intended for controlled experimental environments, including biochemical assays, analytical method development, chromatographic analysis, mass spectrometric quantitation, and in vitro glutathione measurement workflows.

Molecular Information

Compound Type: Tripeptide thiol
Sequence: Glu-Cys-Gly
Molecular Formula: C₁₀H₁₇N₃O₆S
Molecular Weight: 307.33 g/mol
CAS Number: 70-18-8
Purity: 99.80%

Research Applications

In laboratory research, glutathione is used as a defined analyte and reagent in workflows that measure reduced and oxidized glutathione species and in assay systems that monitor glutathione-dependent enzymatic reactions. Published analytical methods describe compound-specific determination of glutathione by HPLC-UV, HPLC with electrochemical detection, LC-MS/MS, fluorescence-based chromatography after derivatization, and capillary electrophoresis.

HPLC-UV assay development for glutathione determination
HPLC with electrochemical detection for glutathione and glutathione disulfide analysis
LC-MS/MS quantitation of reduced and oxidized glutathione
Fluorescence-based chromatographic analysis of derivatized glutathione
Capillary electrophoresis measurement of reduced and oxidized glutathione
Glutathione-dependent enzyme assay workflows, including glutathione S-transferase and glutathione peroxidase measurements